ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influencing factors of transient hypothyroxinemia (THT) and low T3 syndrome (LT3S) in premature infants.</p><p><b>METHOD</b>We have studied 418 premature infants whose gestational age was between 26 and 36 weeks.Serum thyronine (T4), triiodothyronine (T3) and thyrotropin (TSH) of them were detected on the fourteenth day approximately after birth. The patients were divided according to their serum T4, T3 and TSH into 3 groups (transient hypothyroxinemia, low T3 syndrome and normal). Then 20 Perinatal factors which may be associated with THT and LT3S were collected. The factors were analyzed by using Chi-square test and Logistic regression.</p><p><b>RESULT</b>Forty-nine infants were found suffering from THT, 35 infants suffering from LT3S, and 334 infants in normal group. The prevalence rate of THT was 11.7%, and the prevalence rate of LT3S was 8.4%. Among the 20 factors, the factors related to the incidence of THT were male gender (OR = 1.863, 95%CI 0.966-3.594), albumin (OR = 2.401, 95%CI 1.294-4.455), dopamine (OR = 3.295, 95%CI 1.110-9.783) and those related to the incidence of LT3S were male gender (OR = 2.592, 95%CI 1.171-5.736), gestational age ≤ 28 wk (OR = 3.503, 95%CI 1.275-9.627).</p><p><b>CONCLUSION</b>Male gender, albumin and dopamine are perinatal risk factors of THT, meanwhile, male gender and gestational age ≤ 28 wk are perinatal risk factors of LT3S.With the use of risk factors identified in our study, it may be possible to separate infants having the highest risk of THT and LT3S, so as to form optimizing treatment strategies.</p>
Subject(s)
Female , Humans , Infant, Newborn , Male , Case-Control Studies , Dopamine , Euthyroid Sick Syndromes , Blood , Epidemiology , Gestational Age , Hypothyroidism , Blood , Epidemiology , Infant, Premature , Blood , Infant, Premature, Diseases , Blood , Epidemiology , Logistic Models , Risk Factors , Sex Factors , Thyroid Function Tests , Thyronines , Blood , Thyroxine , Blood , Triiodothyronine , BloodABSTRACT
<p><b>OBJECTIVE</b>To evaluate the expression of glucose transporter-4 (GLUT4) mRNA in skeletal muscle and subcutaneous adipose tissues and investigate the mechanism of posttraumatic insulin resistance.</p><p><b>METHODS</b>Sixteen adult male Wistar rats were randomly divided into 2 group (n equal to 8 in each group), i.e., severe traumatic brain injury (TBI) group due to falls from a height and normal control group. Blood glucose and serum insulin were measured at 0.5 h before trauma and 3 h, 24 h, 72 h, 7 d after trauma, respectively. And insulin sensitivity was calculated by insulin activity index (IAI) formula. Skeletal muscle and subcutaneous adipose tissue samples were collected at the same time when blood was sampled. The changes of expression of GLUT4 mRNA were observed using reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Accompanied by the decrease of insulin sensitivity, the expression of GLUT4 mRNA was significantly decreased in adipose tissues at 24 h and 72 h after trauma (P less than 0.01), however, such phenomena did not appear in skeletal muscle samples.</p><p><b>CONCLUSIONS</b>To some extent, the development of posttraumatic insulin resistance is related to the abnormality of transcription activity of GLUT4 gene. Adipose tissues show some difference in the transcriptional level of GLUT4 gene after trauma as compared with skeletal muscle tissues.</p>
Subject(s)
Animals , Male , Rats , Adipose Tissue , Metabolism , Brain Injuries , Metabolism , Glucose Transporter Type 4 , Metabolism , Insulin Resistance , Physiology , Muscle, Skeletal , Metabolism , RNA, Messenger , Metabolism , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To analyze the dynamic change of serum protein S100b in patients with traumatic brain injury and its clinical value in assessing brain damage.</p><p><b>METHODS</b>According to Glasgow coma scale (GCS), 102 cases of traumatic brain injury were divided into mild brain injury group (GCS > or = 13, n = 31, Group A), moderate brain injury group (8 < GCS < 13, n = 37, Group B) and severe brain injury group (GCS < or = 8, n = 34, Group C). Serial S100b concentrations were analyzed by enzyme-linked immunosorbent assay (ELISA) in blood samples taken on admission, 12 h, 24 h, 48 h, 72 h and 7 days after traumatic brain injury.</p><p><b>RESULTS</b>The severe brain injury group showed significantly higher concentration of serum S100b, with earlier increase and longer duration, than the mild and moderate brain injury groups. The patients with higher S100b exhibited lower GCS scores and poor clinical prognosis. The increase in S100b could emerge before clinical image evidence indicated so.</p><p><b>CONCLUSIONS</b>Serum S100b can be used as a sensitive index for assessment and prediction of traumatic brain injury severity and prognosis.</p>
Subject(s)
Adolescent , Adult , Aged , Humans , Middle Aged , Brain Injuries , Blood , Enzyme-Linked Immunosorbent Assay , Nerve Growth Factors , Blood , S100 Calcium Binding Protein beta Subunit , S100 Proteins , BloodABSTRACT
Objective To investigate the clinical significance of procalcitonin(PCT) in early diagnosis of neonatal infections.Methods Rapid hemi-quantitative immunoassay was used to measure PCT levels in 196 hospitalized neonates, who were divided into sepsis group,(local-)infection group, virus-infection group and non-infection group.Results If plasma PCT≥0.5 ?g/L was taken as positive,the positive rates in sepsis group,local-infection,virus-infection,non-infection group were 87.87%,41.66 %,12.0%,11.11%,respectively.The positive rate of sepsis group was significantly higher than that of the other 3 groups(all P